Downloads
Resources available for download include 3-photon data sets and software tools.
Data set: near-simultaneous 2- and 3-photon excitation
Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon
fluorescence movies to compare imaging modalities.
Movies are available for download from the CRCNS data sharing site.
GCaMP6s fluorescence.
910 and 1300 nm excitation.
400 x 200 µm field of view.
~3.6 Hz frame rate.
220-650 µm below the pial surface
of mouse primary visual cortex.
Downloads
Resources available for download include 3-photon data sets and software tools.
Data set: near-simultaneous 2- and 3-photon excitation
Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon
fluorescence movies to compare imaging modalities.
Movies are available for download from the CRCNS data sharing site.
GCaMP6s fluorescence.
910 and 1300 nm excitation.
400 x 200 µm field of view.
~3.6 Hz frame rate.
220-650 µm below the pial surface
of mouse primary visual cortex.
Downloads
Resources available for download include 3-photon data sets and software tools.
Data set: near-simultaneous 2- and 3-photon excitation
Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon
fluorescence movies to compare imaging modalities.
Movies are available for download from the CRCNS data sharing site.
GCaMP6s fluorescence.
910 and 1300 nm excitation.
400 x 200 µm field of view.
~3.6 Hz frame rate.
220-650 µm below the pial surface
of mouse primary visual cortex.
Downloads
Resources available for download include 3-photon data sets and software tools.
Data set: near-simultaneous 2- and 3-photon excitation
Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon
fluorescence movies to compare imaging modalities.
Movies are available for download from the CRCNS data sharing site.
GCaMP6s fluorescence.
910 and 1300 nm excitation.
400 x 200 µm field of view.
~3.6 Hz frame rate.
220-650 µm below the pial surface
of mouse primary visual cortex.
Downloads
Resources available for download include 3-photon data sets and software tools.
Data set: near-simultaneous 2- and 3-photon excitation
Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon
fluorescence movies to compare imaging modalities.
Movies are available for download from the CRCNS data sharing site.
GCaMP6s fluorescence.
910 and 1300 nm excitation.
400 x 200 µm field of view.
~3.6 Hz frame rate.
220-650 µm below the pial surface
of mouse primary visual cortex.
Downloads
Resources available for download include 3-photon data sets and software tools.
Data set: near-simultaneous 2- and 3-photon excitation
Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon
fluorescence movies to compare imaging modalities.
Movies are available for download from the CRCNS data sharing site.
GCaMP6s fluorescence.
910 and 1300 nm excitation.
400 x 200 µm field of view.
~3.6 Hz frame rate.
220-650 µm below the pial surface
of mouse primary visual cortex.
The resource for 3-photon excitation
Updated: Feb 16, 2022
'Improving laser standards for three-photon microscopy' preprint on bioRxiv.
Farinella et al. (2020) characterize third order dispersion and fast pulse energy fluctuations in the output from their Spirit-NOPA. Their study begs the question of whether high order dispersion and pulse-to-pulse energy fluctuations are commonplace with 3-photon lasers.
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