October 2021: Pre-print from the Gibson and Hughes labs.
'Characterization of red fluorescent reporters for dual-color in vivo three-photon microscopy' pre-print on bioRxiv.
Thornton et al. (2021) demonstrate simultaneous red/green imaging in mouse neocortex with GFP and either tdTomato or mScarlet. ~1300 nm drives mixed 2- and 3-photon excitation of tdTomato and mScarlet, with the 2- to 3-photon ration changing across a narrow range of wavelengths from ~1250-1350 nm.