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Downloads

Resources available for download include 3-photon data sets and software tools.

Data set: near-simultaneous 2- and 3-photon excitation

Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon

fluorescence movies to compare imaging modalities.

Movies are available for download from the CRCNS data sharing site.

GCaMP6s fluorescence.

910 and 1300 nm excitation.

400 x 200 µm field of view.

~3.6 Hz frame rate.

220-650 µm below the pial surface

of mouse primary visual cortex.

Downloads

Resources available for download include 3-photon data sets and software tools.

Data set: near-simultaneous 2- and 3-photon excitation

Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon

fluorescence movies to compare imaging modalities.

Movies are available for download from the CRCNS data sharing site.

GCaMP6s fluorescence.

910 and 1300 nm excitation.

400 x 200 µm field of view.

~3.6 Hz frame rate.

220-650 µm below the pial surface

of mouse primary visual cortex.

Downloads

Resources available for download include 3-photon data sets and software tools.

Data set: near-simultaneous 2- and 3-photon excitation

Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon

fluorescence movies to compare imaging modalities.

Movies are available for download from the CRCNS data sharing site.

GCaMP6s fluorescence.

910 and 1300 nm excitation.

400 x 200 µm field of view.

~3.6 Hz frame rate.

220-650 µm below the pial surface

of mouse primary visual cortex.

Downloads

Resources available for download include 3-photon data sets and software tools.

Data set: near-simultaneous 2- and 3-photon excitation

Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon

fluorescence movies to compare imaging modalities.

Movies are available for download from the CRCNS data sharing site.

GCaMP6s fluorescence.

910 and 1300 nm excitation.

400 x 200 µm field of view.

~3.6 Hz frame rate.

220-650 µm below the pial surface

of mouse primary visual cortex.

Downloads

Resources available for download include 3-photon data sets and software tools.

Data set: near-simultaneous 2- and 3-photon excitation

Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon

fluorescence movies to compare imaging modalities.

Movies are available for download from the CRCNS data sharing site.

GCaMP6s fluorescence.

910 and 1300 nm excitation.

400 x 200 µm field of view.

~3.6 Hz frame rate.

220-650 µm below the pial surface

of mouse primary visual cortex.

Downloads

Resources available for download include 3-photon data sets and software tools.

Data set: near-simultaneous 2- and 3-photon excitation

Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon

fluorescence movies to compare imaging modalities.

Movies are available for download from the CRCNS data sharing site.

GCaMP6s fluorescence.

910 and 1300 nm excitation.

400 x 200 µm field of view.

~3.6 Hz frame rate.

220-650 µm below the pial surface

of mouse primary visual cortex.

3photon.org

The resource for 3-photon excitation

October 2021: Pre-print from the Gibson and Hughes labs.

3photon.org
Feb 16, 2022
1 min read

'Characterization of red fluorescent reporters for dual-color in vivo three-photon microscopy' pre-print on bioRxiv.

Thornton et al. (2021) demonstrate simultaneous red/green imaging in mouse neocortex with GFP and either tdTomato or mScarlet. ~1300 nm drives mixed 2- and 3-photon excitation of tdTomato and mScarlet, with the 2- to 3-photon ration changing across a narrow range of wavelengths from ~1250-1350 nm.

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