Resources available for download include 3-photon data sets and software tools.
Data set: near-simultaneous 2- and 3-photon excitation
Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon
fluorescence movies to compare imaging modalities.
Movies are available for download from the CRCNS data sharing site.
910 and 1300 nm excitation.
400 x 200 µm field of view.
~3.6 Hz frame rate.
220-650 µm below the pial surface
of mouse primary visual cortex.
Tool: PSF calculator
Our PSF calculator is a Jupyter notebook that uses vectorial diffraction theory, specifically the equations of Richards & Wolf (1959), to calculate aberration-free microscope point spread functions (PSFs) from three user-defined inputs: wavelength, numerical aperture and refractive index of the immersion medium.