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August 2020: Publication from the Xu lab.

'Three-photon neuronal imaging in deep mouse brain' published in Optica.

Wang & Xu (2020).

Essential reading for anyone interested in 3-photon excitation, this review summarizes the substantial body of work (much of it from the Xu lab) describing the fundamental characteristics, limits and constraints of 3-photon excitation in biological tissues, including

- SNR and the comparison with 2-photon microscopy at different depths.

- Nonlinear and thermal (linear) damage thresholds and power constraints.

- Alternatives to point scanning with 3-photon excitation.

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