August 2020: Publication from the Xu lab.
'Three-photon neuronal imaging in deep mouse brain' published in Optica.
Wang & Xu (2020).
Essential reading for anyone interested in 3-photon excitation, this review summarizes the substantial body of work (much of it from the Xu lab) describing the fundamental characteristics, limits and constraints of 3-photon excitation in biological tissues, including
- SNR and the comparison with 2-photon microscopy at different depths.
- Nonlinear and thermal (linear) damage thresholds and power constraints.
- Alternatives to point scanning with 3-photon excitation.